| 脊神经节感觉神经特异蛋白的分离纯化和鉴定 |
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| 引用本文： | 蒲小平,汪家政,范明. 脊神经节感觉神经特异蛋白的分离纯化和鉴定[J]. 生物物理学报, 1999, 15(2): 315-319 |
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| 作者姓名： | 蒲小平 汪家政 范明 |
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| 作者单位： | 北京医科大学药学院 |
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| 基金项目： | 国家自然科学基金,留学回国人员启动基金 |
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| 摘 要： | 为进一步研究感觉神经特异蛋白２９ＫＤ的结构和功能，我们以兔脊神经节及背根纤维为材料，通过制备匀浆，离子交换层析ＤＥＡＥ－Ｓｅｐｈａｃｅｌ，高压液相凝胶过滤层析分离纯化了感觉神经特异蛋白２９ＫＤ，薄层扫描鉴定纯度为８０％以上，用蛋白质印迹法鉴定了该蛋白，并进行了该蛋白的稳定性观察。为该蛋白作为鉴定感觉神经元及其纤维的标志物，和研究感觉神经元的再生与功能打下了基础
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| 关 键 词： | 脊神经感觉蛋白 纯化 鉴定 |
PURIFICATION AND IDENTIFICATION OF SPECIFIC PROTEIN FROM SPINAL SENSORY GANGLIA |
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| Pu Xiaoping Wang Jiazheng Fan Ming Li Changling. PURIFICATION AND IDENTIFICATION OF SPECIFIC PROTEIN FROM SPINAL SENSORY GANGLIA[J]. Acta Biophysica Sinica, 1999, 15(2): 315-319 |
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| Authors: | Pu Xiaoping Wang Jiazheng Fan Ming Li Changling |
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| Affiliation: | Pu Xiaoping Wang Jiazheng* Fan Ming* Li Changling |
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| Abstract: | A 29ku protein, the sensory neuronal specific protein was isolated and purified from rabbit spinal ganglion and sensory fibers by homogenizing and the soluble extracts followed by ion exchange chromatography using DEAE-Sephacel and gel filtration HPLC. The Purity was estimated to be over 80% by scanning. Western blot showed that it immunoreacts with anti-rabbit 29ku protein monoclonal antibody. The stability has also been studied. 29ku protein is a useful marker for sensory neurons. The purification of it provide a possible means to study the regeneration and function of sensory neurons. |
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| Keywords: | Spinal sensory protein Purification Identification |
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