| 人肝癌细胞瘤QGY-7703的p53基因及其表达研究 |
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| 引用本文： | 王厚义,黄青山,陈翠微,罗祖玉,谢毅,刘建平,苏兆众.人肝癌细胞瘤QGY-7703的p53基因及其表达研究[J].细胞生物学杂志,1997(4). |
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| 作者姓名： | 王厚义 黄青山 陈翠微 罗祖玉 谢毅 刘建平 苏兆众 |
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| 作者单位： | 复旦大学生理学与生物物理学系，复旦大学生理学与生物物理学系，复旦大学生理学与生物物理学系，复旦大学生理学与生物物理学系，复旦大学遗传工程国家重点实验室，复旦大学遗传工程国家重点实验室，Department of Urology Columbia University New York 10032 USA 200433，200433，200433，200433，200433，200433 |
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| 摘 要： | 本文利用等位基因分析,PCR-SSCP,RT-PCR/序列分析,Northern印迹,免疫组织化学,Western印迹,免疫沉淀等方法对人肝癌细胞株QGY-7703的p53基因背景进行了研究,发现17号染色体短臂可能存在等位基因缺失,在p53基因的编码序列上没有发现任何突变,但发现其mRNA和蛋白表达水平很低,表明在QGY-7703细胞中p53基因的低水平表达可能同细胞的恶性程度有关。
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| 关 键 词： | 肿瘤抑制基因p53 PCR/SSCP RT-PCR/序列分析 Northern印迹 免疫 沉淀 |
STUDY ON THE p 53 GENE IN HUMAN HEPATOMA CELL LINE QGY-7703 AND ITS EXPRESSION |
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| WANG Hou Yi HUANG Qing Shan CHEN Cui Wei LUO Zu Yu et al..STUDY ON THE p 53 GENE IN HUMAN HEPATOMA CELL LINE QGY-7703 AND ITS EXPRESSION[J].Chinese Journal of Cell Biology,1997(4). |
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| Authors: | WANG Hou Yi HUANG Qing Shan CHEN Cui Wei LUO Zu Yu |
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| Institution: | Department of Physiology and Biophysics Fudan University Shanghai 200433 |
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| Abstract: | p 53 gene in human hepatoma cell line QGY-7703 was studied wjth following methods: 17 p alleles analysis, PCR-SSCP, RT-PCR/sequencing, Northern blot, immunohistochemistry, Western blot and immunoprecipitation. There may exist allelic loss in the vicinity of p 53 gene on chromosome 17 p. Whereas no any point mutations were found in the coding region of p 53 gene in the cell, Northern blot and immunoprecipitation experiments showed its expre-ssion is quite low, which may be relative with the malignancy of the cell. |
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| Keywords: | Tumor suppressor gene p 53 PCR/SSCP RT-PCR/sequencing Northern blot Immunoprecipitation |
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