Modulation of the number of membrane-bound auxin-binding sites during the growth of batch-cultured tobacco cells |
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| Authors: | D. Vreugdenhil A. Burgers P. A. A. Harkes K. R. Libbenga |
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| Affiliation: | (1) Department of Plant Morphogenesis, Botanisch Laboratorium, Rijksuniversiteit Leiden, Nonnensteeg 3, 2311 VJ Leiden, The Netherlands;(2) Present address: Department of Plant Physiology, Botanisch Laboratorium, Landbouwhogeschool, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands |
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| Abstract: | We studied the modulation of the number of membrane-bound naphthaleneacetic acid (NAA)-binding sites during the growth cycle of tobacco cells in batch culture. Both cell number and specific NAA-binding increased exponentially, but at different rates and for different periods. This caused a characteristic modulation of the number of binding sites per cell during the growth cycle: During the first day of the lag phase this number decreased; in the exponential phase it rose markedly, and in the stationary phase it was constant.Abbreviations MES 4-morpholinoethanesulphonic acid - NAA 1-naphthaleneacetic acid |
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| Keywords: | Auxin-binding site Cell suspension culture Growth cycle Nicotiana |
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