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Mutation of the cohesin related gene PDS5 causes cell death with predominant apoptotic features in Saccharomyces cerevisiae during early meiosis |
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| Authors: | Ren Qun Yang Hui Rosinski Matthew Conrad Michael N Dresser Michael E Guacci Vincent Zhang Zhaojie |
| Institution: | aDepartment of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA bDepartment of Chemical and Petroleum Engineering, University of Wyoming, Laramie, WY 82071, USA cProgram in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA dFox Chase Cancer Center, Basic Science Division, Philadelphia, PA 19111, USA |
| Abstract: | Pds5p is a cohesin related protein. It is required for maintenance of sister chromatid cohesion in mitosis and meiosis. Here we report that pds5-1 causes cell death in yeast Saccharomyces cerevisiae during early meiosis. The pds5-1 caused cell death possesses characteristics of apoptosis and necrosis, including externalization of phosphatidylserine at cytoplasmic membrane, accumulation of DNA breaks, chromatin condensation and fragmentation, nuclei fragmentation, membrane degeneration and cell size enlargement. Our results also suggest that (1) The defect of DNA repair; (2) The production of reactive oxygen species, in pds5-1 mutant are involved in pds5-1 induced cell death. |
| Keywords: | Apoptosis Meiosis Necrosis Pds5p Saccharomyces cerevisiae |
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