Lipases are soluble and active in glycerol carbonate as a novel biosolvent |
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| Authors: | Ou Guangnan He Biyan Yuan Youzhu |
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| Affiliation: | a School of Bioengineering, Jimei University, Xiamen 361021, PR China
b State Key Laboratory of Physical Chemistry of Solid Surfaces and National Engineering Laboratory for Green Chemical Productions of Alcohols-Ethers-Esters, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China |
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| Abstract: | Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pKa value of trichloroacetic acid and transesterification activities of Candida antarctica lipase B and Candida rugosa lipase in glycerol carbonate are similar to those in water, indicating that ionizing and dissociating powers are capable of satisfactorily predicting the biocompatibility of organic solvents for soluble enzymatic systems. |
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| Keywords: | Biosolvent Ionization Dissociation Lipase Activity |
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