The use of microcomputers for the quantitation of light intensity patterns using digitized video signals |
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Authors: | Lott Timothy J; Yang Junghui; Ye Jianhong; Wallace Douglas C |
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Institution: | Department of Biology, and Pediatrics, (Division of 'Medical Genetics), Emory University Atlanta, GA 30322, USA
1Departments of Biochemistry and Pediatrics, (Division of 'Medical Genetics), Emory University Atlanta, GA 30322, USA |
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Abstract: | We have developed an inexpensive yet versatile microcomputer-basedsystem for quantitating light intensity levels in autoradiographs.This system employs a standard video camera interfaced to ananalog-to-digital convertor. A program has been written forthis system which can measure intensities within a defined regionof an autoradiograph, permitting an easy and accurate quantitationof spots or bands of irregular shape.
Received on June 18, 1985; accepted on September 3, 1985 |
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