Studies on cytotoxicity generated in human mixed lymphocyte cultures. III. Natural killer-like cytotoxicity mediated by human lymphocytes with receptors for IgM |
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| Authors: | M G Golightly P D'Amore S H Golub |
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| Affiliation: | 1. Department of Microbiology and Immunology, UCLA School of Medicine, Los Angeles, California 90024 USA;2. Department of Pathology, UCLA School of Medicine, Los Angeles, California 90024 USA;3. Department of Surgery/Oncology, UCLA School of Medicine, Los Angeles, California 90024 USA |
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| Abstract: | Stimulation of human peripheral blood lymphocytes with allogeneic cells in mixed lymphocyte culture (MLC) results in increased NK-like cytotoxicity against K562 targets. The effector cells of this cytotoxicity were shown to include both Fcμ+ and Fcμ? cells, as shown by EAμ rosette separation and by combined rosette formation and single-cell analysis. Peak cytotoxic activity of Fcμ+ cells was found after 3 days of MLC stimulation. The Cytotoxicity against KS62 targets mediated by Fcμ+ cells could not be inhibited at all with alloantigen-bearing cells and could only be partially inhibited with another NK-sensitive target (MOLT- 4). This cytotoxicity could be generated from either Fcγ+ or FCγ? cells. These results indicate considerable heterogeneity of NK-like effectors and their precursors. |
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| Keywords: | To whom correspondence should be addressed: Box 3010 Division of Immunology Duke University Medical Center Durham N.C. 27710. |
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