Isoverbascoside对HL—60细胞的诱导分化和细胞毒作用

Induced-differentiation and cytotoxicity of isoverbascoside on HL-60 cells]

HL-60 cells were treated by isoverbascoside with different time and different concentrations in vitro. The differentiation of HL-60 cells was evaluated by light and electron microscopy to observe morphological changes, by chemiluminence to detect phagocytosis and by tumorigenesis in nude mice to determine malignancy. The cytotoxical effect of isoverbascoside on HL-60 cells was examined by trypan blue excluding staining and electron microscopy. The influence of isoverbascoside on cell cycle was measured by flow cytometry. Granular differentiation of HL-60 cells was induced by isoverbascoside at 20-25 mumol/L within 1-3 days as the results of morphological changes, enhancement of phagocytosis and decreasing of tumorigenesis. Strong cytotoxicity was evidenced in HL-60 cells treated by isoverbascoside at 30-35 mumol/L. HL-60 cells treated by isoverbascoside at 20 mumol/L were delayed at G1 phase at 12 hours and G2/M phase at 72 hours.