Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes |
| |
Authors: | Montembault Emilie Zhang Wei Przewloka Marcin R Archambault Vincent Sevin Emeric W Laue Ernest D Glover David M D'Avino Pier Paolo |
| |
Institution: | 1Department of Pathology, 2Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, and 3Department of Biochemistry, University of Cambridge, Cambridge CB2 1QP, England, UK;4European Bioinformatics Institute, European Molecular Biology Laboratory, Wellcome Trust Genome Campus Hinxton, Cambridge CB10 1SD, England, UK |
| |
Abstract: | Cytokinesis, the final step of cell division, usually ends with the abscission of the two daughter cells. In some tissues, however, daughter cells never completely separate and remain interconnected by intercellular bridges or ring canals. In this paper, we report the identification and analysis of a novel ring canal component, Nessun Dorma (Nesd), isolated as an evolutionarily conserved partner of the centralspindlin complex, a key regulator of cytokinesis. Nesd contains a pectin lyase-like domain found in proteins that bind to polysaccharides, and we present evidence that it has high affinity for β-galactosides in vitro. Moreover, nesd is an essential gene in Drosophila melanogaster, in which it is required for completion of cytokinesis during male meiosis and possibly in female germline cells. Our findings indicate that Nesd is a novel carbohydrate-binding protein that functions together with centralspindlin in late cytokinesis, thus highlighting the importance of glycosylation in this process. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|