小鼠骨髓内皮细胞分泌的细胞因子对造血干/祖细胞增殖的影响

应用小鼠骨髓内皮细胞株细胞传代培养,收集无血清条件培养液（ｍＢＭＥＣ－ＣＭ）,经超滤分成大于１０ｋＤ和小于１０ｋＤ两组分,分别观察两组分的ｍＢＭＥＣ－ＣＭ对小鼠骨髓造血干／祖细胞ＣＦＵ－ＧＭ,ＨＰＰ－ＣＦＣ,ＣＦＵ－Ｅ,ＢＦＵ－Ｅ和ＣＦＵ－Ｍｅｇ的影响。结果表明：含分子量大于１０ｋＤ物质的ｍＢＭＥＣ－ＣＭ的保留液能明显刺激ＣＦＵ－ＧＭ,ＨＰＰ－ＣＦＣ,ＣＦＵ－Ｅ,ＢＦＵ－Ｅ和ＣＦＵ－Ｍｅｇ生长;

The effects of cytokines produced by bone marrow endothelial cells on the regulation of hematopoiesis

In this study, the effects of bone marrow endothelial cell conditioned medium (BMEC-CM) on hematopoietic progenitors and the expression of cytokines mRNA of BMEC were investigated. BMEC-CM was ultrafiltrated in Centriprep-10, an ultrafiltering concentrator. The retentate (MW>10 kD) and filtrate (MW<10 kD) were obtained. The effects of different components of BMEC-CM on the murine hematopoietic progenitors were examined. The results showed that the retentate of BMEC-CM (MW>10 kD) significantly promoted the growth of CFU-GM, CFU-E, BFU-E, CFU-Meg and HPP-CFC, while the growth of CFU-GM, CFU-E, BFU-E, CFU-Meg and HPP-CFC were markedly inhibited by the filtrate of BMEC-CM (MW<10 kD). The effects of BMEC-CM were dose-dependent. The presence of mRNAs for GM-CSF, TGF-beta, bone morphogenetic protein 2A, basic fibroblast growth factor receptor, endothelin-2, macrophage-stimulating 1 (MSP-1), thymosin-beta10, connective tissue growth factor, macrophage inflammatory protein 2 alpha (MIP-2alpha), IL-6, IL-11, IL-13 and IFN-gamma mRNA were successfully detected by Atlas Arrays.