微囊化新生大鼠卵巢组织体外及异体移植

为探讨海藻酸钠-聚左赖氨酸-海藻酸钠(APA)微囊化新生大鼠卵巢组织用于治疗实验性卵巢功能丧失大鼠的可行性,应用高压静电法,用海藻酸钠-聚左赖氨酸-海藻酸钠(APA)生物膜包裹新生大鼠卵巢组织,体外培养微囊,用免疫化学分析法检测雌二醇(E2)、孕酮(P)分泌情况,透射电镜观察卵巢组织形态,并将微囊移植到去势大鼠(切除双侧卵巢的雌性大鼠)腹腔中,检测大鼠血清中雌、孕激素变化情况,同时用阴道涂片观察大鼠动情周期恢复情况,并在不同时间回收观察微囊。结果显示在相同条件下制得的微囊粒径均匀、表面光滑;体外培养条件下持续分泌E2、P;卵巢组织中颗粒细胞发育成为粒性黄体细胞;大鼠腹腔移植微囊后无异常,E2、P水平上升,动情周期未恢复;回收的微囊大部分形态完整。提示用高压静电法制备的APA微囊化新生大鼠卵巢组织能持续稳定释放E2、P,明显改善大鼠卵巢功能,在大鼠体内有良好的生物相容性。

Microencapsulated Newborn Rats Ovaries in Vitro and Allotransplantation

To explore the feasibility of alginate-polylysine-alginate(APA) microencapsulated newborn ratsovaries in treatment of castrated adult female rats.The APA microencapsulated newborn rats ovaries were preparedwith a high-voltage electrostatic system.The microcapsules were cultured in vitro,and the estradiol(E2) andprogesterone(P) secretion were measured periodically,and the ovaries were measured by transmission electronmicroscope periodically.The microcapsules were transplanted to castrated adult female rats,and the E2 and Psecretion were measured periodically.The recovery and maintaining time of the oestrus cycle were carefully observed,and the endocrine function of transplanted ovaries was determined by measuring serum E2 and P.The transplantedmicrocapsules were recovered at intervals.The microcapsules prepared in the same condition of high-voltageelectrostatic system were round and homogeneous.The E2,P secreted by microencapsulated newborn rats ovarieswere shown the permeate of the membrane of APA microcapsules,and the develop of granulose cells in ovarieswere founded by transmission electron microscope in vitro.After microcapsules were transplanted,the level of E2,P in castrated adult female rats were raised gradually,but the oestrus cycle were not resumed.The majority ofrecovered APA microcapsules were intact,and no evidence of immunological reaction in terms of fibrosis.The E2and P were secreted continually and steadily by the APA microencapsulated newborn rats ovaries prepared by high-voltage electrostatic system.The ovarian function of castrated adult female rats were improved by APA microcapsules,and the microcapsules have good biocompatibility in vivo.