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BLI-MS: Combining biolayer interferometry and mass spectrometry |
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| Authors: | Vincent Jung Kévin Roger Cerina Chhuon Louise Pannetier Joanna Lipecka Josué Sulub Gomez Pascal Chappert Alain Charbit Ida Chiara Guerrera |
| Institution: | 1. Proteomics Platform Necker, Université de Paris - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris, France;2. Université de Paris, Sorbonne Paris Cité, INSERM U1151-CNRS UMR 8253, Institut Necker-Enfants Malades, Paris, France;3. Université de Paris, Sorbonne Paris Cité, INSERM U1151-CNRS UMR 8253, Institut Necker-Enfants Malades, Paris, France
INSERM U955, Institut Mondor de Recherche Biomédicale (IMRB), Université Paris-Est Créteil (UPEC), Créteil, France |
| Abstract: | Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to identify the proteins interacting with the bait. We provide for the first time the proof of concept of the feasibility of BLI-MS in complex biological mixtures. |
| Keywords: | biolayer interferometry mass spectrometry molecular interaction |