Human peripheral blood mononuclear cells: A review of recent proteomic applications |
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| Authors: | Michal Alexovič Joshua Raoul Lindner Peter Bober Rémi Longuespée Ján Sabo Katarina Davalieva |
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| Affiliation: | 1. Department of Medical and Clinical Biophysics, Faculty of Medicine, University of P.J. Šafárik in Košice, Košice, Slovakia;2. Department of Clinical Pharmacology and Pharmacoepidemiology, Heidelberg University Hospital, Heidelberg, Germany;3. Research Centre for Genetic Engineering and Biotechnology, “Georgi D Efremov”, Macedonian Academy of Sciences and Arts, Skopje, North Macedonia |
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| Abstract: | Human peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune-related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in-gel, in-solution and on-filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates. |
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| Keywords: | human peripheral blood mononuclear cells (PBMCs) mass spectrometry protein biomarkers proteomics sample preparation |
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