Compared roles of glucose, galactose and fructose as glycogen precursors during the acute response to insulin in cultured rat foetal hepatocytes |
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Authors: | P Menuelle H M'zali N Forest C Plas |
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Institution: | Laboratoire Interactions Cellulaires, UER Odontologie, Université Paris, France. |
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Abstract: | 1. The efficiency of the contribution of hexoses to basal- and stimulated-glycogenesis, when studied in cultured 18 day-old rat foetal hepatocytes in the presence of glucose, was as follows: galactose greater than glucose greater than fructose. 2. Glucose deprivation had opposite effects on the contributions of 14C]galactose (decreased) and 14C]fructose (increased) to glycogenesis, which occurred independently of insulin and were reversed by glucose concentrations as low as 30-100 microM. 3. The stimulation of glycogenesis by insulin measured with 14C]glucose (3.2-fold) was superior to that obtained with either 14C]galactose or 14C]fructose (2.7-fold in both cases), which revealed a specific beneficial effect of insulin on glucose contribution. |
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