Nuclease Rsn from Rhizopus stolonifer: specificity and mode of action |
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Authors: | Rangarajan E S Shankar V |
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Institution: | Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India. |
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Abstract: | Nuclease Rsn from Rhizopus stolonifer catalyzes the hydrolysis of ss- and dsDNA in a ratio of approximately 2:1. Time course of 3' and 5' terminal analysis of the hydrolytic products of ss- and dsDNA showed that nuclease Rsn does not show any strict base preference and cleaves DNA in a non-specific manner. Moreover, separation of the hydrolytic products of ss- and dsDNA in the presence of Mg2+, Mn2+ or Co2+ showed the predominance of tetra-, tri-, and dinucleotides followed by mononucleotides, suggesting an endo mode of action. |
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Keywords: | Nuclease Rsn Base specificity Endonuclease Rhizopus stolonifer |
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