| 新型东亚钳蝎小分子成分的生化性质及其对大鼠心室肌细胞Ito的作用 |
| |
| 引用本文： | 童青春,张翼,周兆年,吉永华.新型东亚钳蝎小分子成分的生化性质及其对大鼠心室肌细胞Ito的作用[J].生物化学与生物物理学报,1999(3). |
| |
| 作者姓名： | 童青春 张翼 周兆年 吉永华 |
| |
| 作者单位： | 中国科学院上海生理所，上海生命科学研究中心 |
| |
| 基金项目： | 国家杰出青年基金,中科院择优资助项目 |
| |
| 摘 要： | 分离纯化获得了两个东亚钳蝎小分子活性多肽ＢｍＰ０２和ＢｍＰ０３。经质谱鉴定二者皆为单一峰，分子量分别为２．９５０ｋＤ和２．９３５ｋＤ。ＢｍＰ０２的氨基酸序列已被测定。用全细胞膜片箝方法记录到ＢｍＰ０２对大鼠心室肌细胞短暂外向钾电流（Ｉｔｏ）具有明显的抑制作用（ｎ＝５），冲洗后可部分恢复，且其抑制活性具有浓度依赖性和电压非依赖性。进一步研究表明，ＢｍＰ０２可影响短暂外向钾通道的失活化和恢复过程，但却不影响其激活过程
|
| 关 键 词： | 蝎毒素 钾通道 生化分离 全细胞膜片箝 |
The Characterizations and Blocking Effects on Ito of Rat Ventricular Myocyte of New
Minipeptides from Buthus martensi Karsch |
| |
| TONG Qing Chun ,ZHANG Yi ,ZHOU Zhao Nian and JI Yong Hua.The Characterizations and Blocking Effects on Ito of Rat Ventricular Myocyte of New
Minipeptides from Buthus martensi Karsch[J].Acta Biochimica et Biophysica Sinica,1999(3). |
| |
| Authors: | TONG Qing Chun ZHANG Yi ZHOU Zhao Nian and JI Yong Hua |
| |
| Institution: | TONG Qing Chun 1,2,ZHANG Yi 1,ZHOU Zhao Nian 1 and JI Yong Hua 1,2 |
| |
| Abstract: | Two minipeptides (BmP02 and BmP03) with M.W. 2.950 kD and
2.935 kD determined respectively by electrospray ionization mass spectrum, were purified from
the venom of Buthus martensi Karsch by an opportune procedure on cation exchange
column chromatography and repeated HPLC. The amino acid sequence of BmP02 had been
determined. Using whole cell patch clamp, BmP02 was studied on rat ventricular myocyte. The
results showed that BmP02 inhibited the transient outward current (Ito) of rat ventricular
myocytes with partial recovery after washout ( n =5) and its inhibition had concentration
dependence and voltage independence. Further study showed that BmP02 affected both the
inactivation and recovery kinetics, but not activation kinetics of Ito channel. |
| |
| Keywords: | Scorpion toxin potassium channel biochemical purification whole cell patc clamp |
| 本文献已被 CNKI 等数据库收录！ |
