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Gradient-enhanced 3D NOESY-HMQC spectroscopy |
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| Authors: | Geerten W Vuister Rolf Boelens Robert Kaptein Maurits Burgering Peter C M van Zijl |
| Institution: | (1) Bijvoet Center for Biomolecular Research, Department of Chemistry, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands;(2) Department of Pharmacology, Georgetown University Medical School, 4 Research Court, 20850 Rockville, MD, USA;(3) Present address: Laboratory of Chemical Physics, Building 2, NIDDK, National Institutes of Health, 20892 Bethesda, MD, USA;(4) Department of Radiology, 110 MRI Building, Johns Hopkins School of Medicine, 600 North Wolfe Street, 21205 Baltimore, MD, USA |
| Abstract: | Summary A gradient-enhanced 3D NOESY-HMQC experiment is presented and applied to15N-labeled Mnt repressor (1–76) in1H2O. Both coherence selection and1H2O suppression are achieved using gradients. A singlescan experiment can be recorded for each time increment, which greatly reduces recording time with respect to conventional methods using phase cycling. This can be of use for kinetic studies and for relatively unstable molecules. |
| Keywords: | Gradient-enhanced NMR Mnt repressor 3D NMR NOESY-HMQC |
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