Characterization of cluster-assembled nanostructured titanium oxide coatings as substrates for protein arrays |
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Authors: | Roberta Carbone Marzia De Marni Andrea Zanardi Simone Vinati Emanuele Barborini Lorenzo Fornasari Paolo Milani |
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Institution: | aTethis, Via Russoli 3, 20143 Milano, Italy;bCongenia–Genextra Group, Piazzetta Bossi 4, 20121 Milano, Italy;cCIMAINA and Dipartimento di Fisica, Università di Milano, Via Celoria 16, 20133 Milan, Italy |
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Abstract: | Protein microarray technologies are rapidly expanding to fulfill current needs of proteome discovery for disease management. Nanostructured materials have been shown to present interesting features when used in biological settings: nanostructured titanium oxide film (ns-TiOx), synthesized by supersonic cluster beam deposition (SCBD), has recently emerged as a biocompatible substrate in different biological assays. The ns-TiOx surface is characterized by a morphology at the nanoscale that can be tuned to modulate specific biomolecule–material interactions. Here we present a systematic characterization of ns-TiOx coatings as protein binding surfaces, comparing their performances with those of most common commercial substrates in protein and antibody microarray assays. Through a robust statistical evaluation of repeatability in terms of coefficient of variation (CV) analysis, we demonstrate that ns-TiOx can be used as reliable substrate for biochips in analytical protein microarray application. |
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Keywords: | Protein microarrays Nanobiotechnology Titanium oxide (TiOx) Biochips |
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