Scanning molecular sieve chromatography of interacting protein systems. Simulation of large zone behavior for self-associating solutes undergoing rapid chemical equilibration under kinetic control |
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| Authors: | P W Chun M C Yang |
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| Institution: | Department of Biochemistry and Molecular Biology, College of Arts and Sciences, University of Florida, Gainesville, Florida 32610, USA |
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| Abstract: | Theoretical large zone reaction boundaries for molecular sieve chromatography have been simulated by computer for a self-associating solute undergoing rapid chemical equilibration under kinetic control. These patterns show that the kinetically-controlled reaction rate between the mobile and stationary phases is the principal determinant of the elution boundary profile in molecular sieve chromatography. The overall chemical reaction rate in the mobile phase was found to have a much greater role in a rapidly equilibrating system than did the effect of axial dispersion within the gel matrix. |
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| Keywords: | Author to whom correspondence should be addressed |
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