Marking bark beetle parasitoids within the host plant with rubidium for dispersal studies |
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| Authors: | E Hougardy P Pernet M Warnau J Delisle & J-C Grégoire |
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| Institution: | Biologie des Communautés animales CP 160/12, UniversitéLibre de Bruxelles, 50 av. F.D. Roosevelt, B-1050 Bruxelles, Belgium;;Laboratoire de Biologie Marine, UniversitéLibre de Bruxelles, 50 av. F.D. Roosevelt, B-1050 Bruxelles, Belgium;;Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du P.E.P.S., PO Box 3800, Sainte-Foy, Quebec, G1V 4C7, Canada |
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| Abstract: | A technique using rubidium chloride (RbCl), a trace element, as internal label is proposed for marking hymenopteran parasitoids attacking a concealed host, Ips typographus L. (Coleoptera: Scolytidae). RbCl was introduced directly into spruce via the vascular system using glass tubing. RbCl passed through the food chain and was detected at the parasitoid level by electrothermal atomic absorption spectroscopy (EAAS) or flame emission spectroscopy (FES). Mark persistency until day 8 after emergence was tested in labelled Rhopalicus tutela (Walker) (Hymenoptera: Pteromalidae). The mark decreased with time only in fed females, probably due to excretion and/or egg resorption. The proportion of marked males did not vary with time or treatment. |
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| Keywords: | Ips typographus Rhopalicus tutela Coeloides bostrichorum mark-capture tree infusion EAAS FES Coleoptera Scolytidae Hymenoptera Pteromalidae Braconidae |
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