Extra proton translocation and membrane potential generation--universal properties of cytochrome bc1/b6f complexes reconstituted into liposomes |
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| Authors: | E C Hurt N Gabellini Y Shahak W Lockau G Hauska |
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| Institution: | 2. Institut für Botanik, Universität Regensburg, D-8400 Regensburg, West Germany;1. Biochemistry Department, Weizmann Institute of Science, Rehovot, 76100, Israel |
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| Abstract: | Isolated cytochrome complexes from different sources like beef heart mitochondria, spinach chloroplasts, cyanobacteria, and photosynthetic bacteria were incorporated into liposomes by sonication as revealed by sucrose density gradient centrifugation and electron microscopy. The reconstituted cytochrome complexes show suppressed rates of quinol-cytochrome c/plastocyanin oxidoreduction which can be stimulated by ionophores and uncouplers. In addition, extra proton translocation out of the vesicles and membrane potential generation during electron transport were observed, suggesting a universal mechanism of electron and proton transport through all the tested cytochrome complexes. |
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| Keywords: | To whom correspondence should be addressed |
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