Alkylation and cleavage of a DNA fragment with trioligonucleotide reagent |
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Authors: | Oshevski S I |
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Affiliation: | Institute of Cytology and Genetics of the Russian Academy of Sciences, Novosibirsk, Russia. oshevski@bionet.nsc.ru |
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Abstract: | A possibility of site-directed chemical modification of a ssDNA fragment with "trioligonucleotide reagent" (TOR), consisting of a central oligonucleotide derivative carrying N-(2-chloroethyl)-N-(p-formylphenyl)-N-propyl-N-3-ydeneamino groups at both 5'- and 3'-thiophosphate ends and two border derivatives with 4-carbohydrazidephenyl groups at their 3'- and 5'-phosphate ends, respectively, is shown. Products of site-directed fragment cleavage, more abundant than the alkylation products, were found at 50 degrees C. The overall level of DNA modification by TOR reached 30% at a small excess of the oligonucleotide derivatives. |
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