| 一种适合膜片钳单通道记录的大鼠DRG神经元急性分离方法 |
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| 引用本文： | Zhang CB,Yu XL,Pan ZQ,Shao CJ,Cao JL. 一种适合膜片钳单通道记录的大鼠DRG神经元急性分离方法[J]. 中国应用生理学杂志, 2011, 27(4): 510-512 |
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| 作者姓名： | Zhang CB Yu XL Pan ZQ Shao CJ Cao JL |
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| 作者单位： | 徐州医学院省麻醉学重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(81070888); 徐州医学院院长专项研究基金(09KJZ16) |
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| 摘 要： | 目的：建立一种适合膜片钳单通道记录的脊髓背根神经节神经元急性分离方法。方法：用酶消化和机械分离相结合的方法急性分离大鼠DRG神经元。结果：用本方法分离的DRG细胞容易形成较高的封接电阻（〉5GΩ）,降低了噪音干扰,可记录到pA级的单通道电流。结论：本方法急性分离的DRG神经元适合单通道膜片钳实验研究。
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| 关 键 词： | 膜片钳 单通道 DRG神经元 急性分离 |
A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel |
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| Zhang Cheng-Biao,Yu Xiao-Lu,Pan Zhi-Qiang,Shao Cui-Jie,Cao Jun-Li. A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel[J]. Chinese journal of applied physiology, 2011, 27(4): 510-512 |
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| Authors: | Zhang Cheng-Biao Yu Xiao-Lu Pan Zhi-Qiang Shao Cui-Jie Cao Jun-Li |
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| Affiliation: | ZHANG Cheng-biao,YU Xiao-lu,PAN Zhi-qiang,SHAO Cui-jie,CAO Jun-li(Jiangsu Provincial Key Laboratory of Anesthesiology,Xuzhou Medical College,Xuzhou 221002,China) |
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| Abstract: | Objective： To estsblish a method of acutely isolating dorsal root ganglion （DRG） neurons for patch clamp study of singlechannel. Methods： DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing. Results： The acutely isolated DRG ceils were easy to form the higher sealing resistance （ 〉 5G~）, which lowered noise level,so that pA-level single channel currents could be recorded. Conclusion： The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel. |
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| Keywords: | patch-clamp single channel dorsal root ganglion neuron acute isolation |
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