| NOS抑制剂对甲醛炎性痛诱导的大鼠痛反应及脊髓神经元凋亡的影响 |
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| 作者姓名： | Chu SC Hu YY Li QJ Li HN Li WB |
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| 作者单位： | 河北医科大学基础医学院病理生理学教研室; |
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| 基金项目： | 河北省科技攻关课题(06276102D-40) |
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| 摘 要： | 目的：观察甲醛炎性痛是否可诱导脊髓神经元凋亡以及一氧化氮（NO）对甲醛炎性痛诱导的大鼠痛反应以及脊髓神经元凋亡的影响。方法：采用行为学方法观察大鼠自发痛反应,流式细胞术检测脊髓神经元凋亡率。结果：与正常大鼠比较,甲醛炎性痛可诱导大鼠脊髓神经元凋亡率明显增加,于注射甲醛后3d时最为明显。预先鞘内注射NOS抑制剂L-NAME可剂量依赖性抑制足底注射甲醛诱导的大鼠第一相和第二相痛反应,并可剂量依赖性抑制足底注射甲醛诱导的脊髓神经元凋亡过程;正常大鼠鞘内注射L-Arg也可诱发出伤害性反应和脊髓神经元凋亡。结论：甲醛炎性痛可诱导脊髓神经元发生凋亡,于注射甲醛后3d神经元凋亡最为明显;炎性痛诱导的NO产生增多促进了脊髓伤害性信息传递过程,并在炎性痛诱导的脊髓神经元凋亡过程中发挥促进作用。
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| 关 键 词： | 甲醛炎性痛 脊髓 神经元凋亡 |
Effects of nitric oxide on spontaneous pain reaction and neuronal apoptosis in the spinal cord of rats induced by formalin inflammatory pain |
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| Chu SC,Hu YY,Li QJ,Li HN,Li WB.Effects of nitric oxide on spontaneous pain reaction and neuronal apoptosis in the spinal cord of rats induced by formalin inflammatory pain[J].Chinese Journal of Applied Physiology,2011,27(3):372-375. |
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| Authors: | Chu Sai-chun Hu Yu-yan Li Qing-jun Li Hui-na Li Wen-bin |
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| Institution: | Department of Pathophysiology, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050017, China. |
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| Abstract: | Objective:To observe whether formalin inflammatory pain can induce neuron apoptosis in rats spinal cord or not and the effects of nitric oxide on the spontaneous pain reaction and neuron apoptosis in the spinal cord of rats with formalin inflammatory pain.Methods: Formalin-induced paw licking time was used to reflect the degree of spontaneous pain of rats,and the flow cytometry was used to detecte neuron apoptosis rate of spinal cord.Results: Compared with control group,the apoptosis ratio of spinal neuron ... |
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| Keywords: | formalin inflammatory pain spinal cord neuron apoptosis |
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