| 细胞外信号调节激酶在丹参单体IH764-3诱导肝星状细胞凋亡中的作用 |
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| 作者姓名： | Fang SM Li CS An JY Dun ZN Yao DM Liu L Zhang XL |
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| 作者单位： | 河北医科大学第二医院消化科河北省消化病重点实验室河北省消化病研究所;河北顺平县医院内科; |
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| 基金项目： | 河北省中医药管理局科研课题(2007061); 河北省科技厅资助项目(09966107D) |
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| 摘 要： | 目的：探讨丹参单体1H764-3对H202刺激的肝星状细胞（HSCs）增殖、凋亡等细胞行为的影响及细胞外信号调节激酶,（ERKt）在其中的调节作用。方法：应用体外细胞培养技术,采用直接细胞计数法、0H．胸腺嘧啶核苷（0H-TdR）掺入法测定HSCs增殖;透射电镜、膜联蛋白（Annexin-V）／磺化丙啶（PI）双标记流式细胞术测定nscs凋亡;分别应用Western blot和逆转录-聚合酶链反应（RT-PCR）技术测定ERK1蛋白及其mRNA的表达。结果：①H202具有刺激HSC8增殖的作用;②丹参单体IH764-3剂量依赖性抑制也02刺激的HSCs增殖;③Annexin-V／PI检测显示,10mg／L,20mg／L,30mg／L及40mg／LIH764-3干预48h后各组凋亡率分别为6．35％、9．28％、15．10％、19．69％,而H2O2组为2．30％;30ms／L IH764-3干预HSCs不同时间（12h、24h、48h）的凋亡率分剐是6．73％、10．34％、15．10％,呈时间依赖性;④丹参单体IH764-3干预组,HSCs的ERK1蛋白及其mRNA表达下调。结论：丹参单体IH764-3可以抑制HSCs增殖并诱导其凋亡;这种作用与其抑制ERK1蛋白和ERK1 mRNA表达有关。
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| 关 键 词： | 肝星状细胞 细胞外信号调节激酶 凋亡 丹参单体 IH764-3 过氧化氢 |
The role of extracellular signal-regulated kinase in induction of apoptosis with salvia miltiorrhiza monomer IH764-3 in hepatic stellate cells |
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| Fang SM,Li CS,An JY,Dun ZN,Yao DM,Liu L,Zhang XL.The role of extracellular signal-regulated kinase in induction of apoptosis with salvia miltiorrhiza monomer IH764-3 in hepatic stellate cells[J].Chinese Journal of Applied Physiology,2011,27(4):402-406. |
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| Authors: | Fang Shu-Ming Li Chun-Sheng An Jun-Yan Dun Zhi-Na Yao Dong-Mei Liu Lei Zhang Xiao-Lan |
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| Institution: | FANG Shu-ming1,LI Chun-sheng2,AN Jun-yan1,DUN Zhi-na1,YAO Dong-mei1,LIU lei1,ZHANG Xiao-lan1(1.Department of Gastroenterology,the Second Hospital of Hebei Medical University,Hebei Key Laboratory of Gastroenterology,Hebei Institute of Gastroenterology,Shijiazhuang 050000,2.Department of Internal Medicine,Shunping County Hospital,Shunping 072250,China) |
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| Abstract: | Objective: To explore the effect of Salvia miltiorrhiza monomer IH764-3 on apoptosis in hydrogen peroxide(H2O2)-stimulated hepatic stellate cells(HSCs).Methods: HSCs were cultured in medium with different IH764-3 doses(10 mg/L,20 mg/L,30 mg/L,40 mg/L) and without IH764-3.Direct cell count,3H-thymidine incorporation,Annexin-V/Propidium Iodide double-labeled flow cytometry, TUNEL and transmission electron microscopy were employed to estimate the influence of IH764-3 on proliferation and apoptosis of HSCs.The ... |
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| Keywords: | hepatic stellate cell extracellular signal-regulated kinase apoptosis salvia miltiorrhiza monomer IH764-3 hydrogen peroxide |
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