| S1P对心肌细胞的保护作用研究 |
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| 引用本文： | Yuan L,Zhang WJ,Zhang DD,Zhao CY. S1P对心肌细胞的保护作用研究[J]. 中国应用生理学杂志, 2011, 27(3): 320-323 |
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| 作者姓名： | Yuan L Zhang WJ Zhang DD Zhao CY |
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| 作者单位： | 漯河医学高等专科学校;吉林大学白求恩医学院生理学教研室; |
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| 摘 要： | 目的：探究1-磷酸鞘氨醇（S1P）对缺氧／复氧乳鼠心肌细胞的保护作用及其分子机制。方法：在大鼠乳鼠心肌细胞原代培养基础上,应用液体石蜡覆盖法制备心肌细胞缺氧／复氧模型,采用流式细胞术PI染色法和流式细胞术罗丹明123染色法检测S1P对缺氧／复氧心肌细胞的细胞凋亡和线粒体膜电位的影响;Western Blot分析法检测S1P作用后的心肌细胞p-Akt1蛋白水平变化,并且观察PI3K（磷脂酰肌醇3-激酶）阻断剂渥曼青霉素（wommamin）对S1P上述作用的影响。结果：在S1P的影响下,缺氧／复氧心肌细胞的凋亡率显著下降（P〈0．01）,线粒体膜电位的去极化被明显抑制（P〈0．05）,p-Akt1水平明显升高（P〈0．01）,wormannin能够部分阻断S1P的上述效应。结论：S1P能够显著抑制缺氧／复氧引起的心肌细胞凋亡,其机制可能与S1P激活PDK-Akt信号通路进而稳定线粒体膜电位有关。
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| 关 键 词： | 1-磷酸鞘氨醇 缺氧／复氧 Akt 线粒体膜电位 渥曼青霉素 |
The study on protective effect of sphingosine-1-phosphate in cardiomyocytes |
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| Yuan Lei,Zhang Wen-jie,Zhang Duo-duo,Zhao Chun-yan. The study on protective effect of sphingosine-1-phosphate in cardiomyocytes[J]. Chinese journal of applied physiology, 2011, 27(3): 320-323 |
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| Authors: | Yuan Lei Zhang Wen-jie Zhang Duo-duo Zhao Chun-yan |
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| Affiliation: | YUAN Lei1,2,ZHANG Wen-jie2,ZHANG Duo-duo2,ZHAO Chun-yan2(1.Department of Physiology,Luohe Medical College,Luohe 462002,2.Department of Physiology,Norman Bethune Medical College,Jilin University,Changchun 130021,China) |
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| Abstract: | Objective:To explore the mechanism of protective effect of Sphingosine-1-phosphate(S1P) in cultured neonatal rat cardiomyocytes during simulated hypoxia/reoxygenation.Methods: On the basis of culturing neonatal rat cardiomyocytes,the model of hypoxia-reoxygennation was built by using method of Liquid Paraffin covering,the impact of S1P on apoptosis and p-Akt and mitochondrial membrane potential were studied by using method of Propidine Iodide staining and Western blot and Rhodamine123 staining.Results: S1P ... |
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| Keywords: | sphingosine-1-phosphate hypoxia-reoxygennation Akt mitochondrial membrane potential wormannin |
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