氧化应激在高原重体力劳动过程中急性高原反应发生中的作用

目的：观察氧化应激在高原重体力劳动过程中急性高原反应（AHAR）发生中的作用。方法：由低海拔（1500m）快速进入高原（3700m）并从事重体力劳动的男性官兵96名,年龄18～35岁。根据AHAR症状评分,分为重度AHAR组（A组,n=24）、轻中度AHAR组（B组,n=47）和无AHAR组（C组,n=25）,在该高度逗留50d后下撤前及返回低海拔（1500m）后12h、15d分别测定血清8．异前列腺素F2a（8-iso-PGF2a）、超氧化物歧化酶（SOD）、丙二醛（MDA）,并与低海拔（1500m）50名健康官兵（D组）比较。结果：A组血清8-iso-PGF2a、MDA[分别为（9．53±0．47）μg／L、（8．91±0．39）μmol／L]水平显著高于B组[分别为（8．34±O．42）μg／L、（7．31±0．32）μmol／L]、C组[分别为（7．02±0．48）μg／L、（6．41±0．23）μmol／L和D组[分别为（5．13±0．56）μg／L、（5．48±0．33）μmol／L]（均P〈0．01）,SOD（52．08±3．44）μ／mL水平显著低于B组（62．27±2．54）μ/mL、C组（71．99±3．35）μ/mL和D组（80．78±3．44）μ/mL,（均P〈0．01）,B组与c组之间和C组与D组之间亦有显著性差异（均P〈0．01）。海拔3700mAHAR总计分与血清8-iso-PGF2α、ⅣⅡ）A呈显著正相关（均P〈0．01）,与血清SOD显著负相关（P〈0．01）;8-iso-PGF2α、MDA与SOD显著负相关（均P〈0．01）。海拔3700m50d,血清8-iso-PGF2α、MDA水平显著高于,SOD水平显著低于海拔1500m12h、15d和D组（均P〈0．01）,海拔1500m12h与15d之间有显著性差异（均P〈0．01）,海拔1500m 15d与D组之间无显著性差异。结论：人体在高原低氧并重体力时氧化应激和氧化．抗氧化失衡与AHAR的发病和程度有密切关系,氧化应激和氧化．抗氧化失衡越严重,AHAR越重。返回低海拔后12h有显著改善,15d恢复到正常水平。

Effect of oxidative stress in development of acute high altitude response during the process of strong physical work at high altitude

Objective： To assess the effect of oxidative stress in development of acute high altitude response （AHAR） during the process of strong physical work at high altitude and its change after return to lower altitude. Methods： Ninety-six officers and soldiers of rapid entering into high altitude （3 700 m） with strong physical work were analyzed, all subjects were male, aged 18 - 35 years. According to the symptomatic scores of AHAR were divided into 3 groups： severe AHAR （group A, n = 24）, mild AHAR （group B, n = 47） and without AHAR （group C, n = 25）. Levels in serum 8-iso prostaglandinF2α（8-iso-PGF2α）, superoxide dismutase（SOD） and malonaldehyde（MDA） were measured at higher altitude stayed 50 d and after return to lower altitude （1 500 m） 12 h and 15 d,and 50 healthy volunteers （group D） at 1 500 m altitude served as controll. Results： Levels of serum 8-iso-PGF2α and MDA[ （ 9.53 ± 0.47） μg/L, （8.91 ± 0.39 ） μmol/L] were significantly higher in group A than those in group B [ （8.34 ± 0.42）μg/L, （7.31 ± 0.32）μmol/L] , group C [ （7.02 ± 0.48）μg/L, （6.41 ±0. 23）μmol/L] and group D [ （5.13 ± 0.56）μg/L, （5.48 ± 0.33）μmol/L], （all P 〈 0.01）, and serum SOD [ （52.08 ± 3.44）μmol/L was significantly lower in group A than that in group B [62.27 ± 2.54）μ/ml], group C [ （71.99 ± 3.35）μ/ml] and group D [ （80.78 ± 3.44）μ/ ml] （all P 〈 0.01）, there were signitlcantl differences between group B and C, C and D （all P 〈 0.01） . At altitude 3 700 m 50 d,AHAR scores was positively correlated with serum 8-iso-PGF2a and MDA （all P 〈 0.01）, negatively correlated with SOD（ P 〈 0.01）. Serum 8-iso- PGF2a and MDA were negatively correlated with SOD （all P 〈 0.01）. Levels of serum 8-ise-PGF2α and MDA were significantly higher at altitude of 3 700 m 50 d than those at altitude of 1 500 m 12 h, 15 d in group D （all P 〈 0.01）, and serum SOD was significantly lower than that at 1 500 m 12 h, 15 d in group D （all P 〈 0.01）, there were significantly difference between at 1 500 m 12 h and 15 d （all P 〈 0.01）, there were no difference between at 15 d in group D（all P 〉 0.05）. Conclusion The more serious of oxidative stress and oxidative/antioxidative imbalance,the more serious of AHAR, oxidative stress and oxidative/antioxidative imbalance may be involved in the development of AHAR. The changes were obviously improved after return to lower altitude 12 h, and recovered to normal after 15 d.