| TOLL样受体4在哮喘大鼠气道平滑肌细胞增殖、凋亡中的作用 |
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| 作者姓名： | Wei JH Mo BW Huang JW |
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| 作者单位： | 桂林医学院附属医院呼吸内科; |
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| 基金项目： | 国家自然科学基金资助项目(30760083); 广西科学研究与技术开发计划课题(桂科攻0719006-2-8); 广西卫生厅(重200732,重200976); 桂林市科学研究与技术开发计划课题(20080320-1); 广西青年科学基金(桂科青0991085); 桂林医学院2009年硕士研究生科研创新项目(555); 广西医疗卫生自筹课题(Z2008266) |
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| 摘 要： | 目的:探讨Toll样受体4(TLR4)在哮喘状态下气道平滑肌细胞(ASMCs)增殖、凋亡中的作用.方法:建立哮喘大鼠模型,分离、培养哮喘大鼠气道平滑肌细胞,应用小分子RNA干扰技术、脂质体转染法进行小分子RNA-TLR4的转染、MTT,法检测细胞增殖、TUNNEL法检测细胞凋亡情况、逆转录聚合酶链式反应(RT-PCR)...
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| 关 键 词： | Toll样受体4 支气管哮喘 增殖 调亡 |
Role of toll-like receptor 4 in the asthmatic rat airway smooth muscle cells proliferation and apoptosis |
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| Wei JH,Mo BW,Huang JW.Role of toll-like receptor 4 in the asthmatic rat airway smooth muscle cells proliferation and apoptosis[J].Chinese Journal of Applied Physiology,2011,27(3):284-288. |
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| Authors: | Wei Jiang-hong Mo Bi-wen Huang Jian-wei |
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| Institution: | WEI Jiang-hong,MO Bi-wen,HUANG Jian-wei (Department of Respiratory Medicine,Hospital affiliated Guilin Medical College,Guilin 541001,China) |
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| Abstract: | Objective:To explore the role of Toll like receptor 4(TLR4) in the asthmatic rat airway smooth muscle cell(ASMCs) proliferation and apoptosis.Methods: Established rat model of asthma,isolated and cultured rat ASMCs in asthma,using methods of small molecule RNA interference technology and lipofection method,for small molecule RNA-TLR4 transfection,detected proliferation of ASMCs by MTT minim colorimetry,apoptosis of ASMCs by TUNNEL,the expression of TLR4 protein and mRNA were detected by Western blot and RT-... |
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| Keywords: | Toll like receptors 4 asthma proliferation apoptosis |
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