| 盐酸戊乙奎醚（长托宁）抑制脑缺血／再灌注时谷氨酸的释放及受体机制研究 |
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| 作者姓名： | Shang Y Gu PF Shang Y Li Y |
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| 作者单位： | 辽宁医学院附属一院麻醉科;沈阳药科大学生理研究室;解放军第463医院麻醉科;哈尔滨医科大学附属一院麻醉科; |
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| 摘 要： | 目的：观察盐酸戊乙奎醚对全脑缺血／再灌注大鼠易损区海马谷氨酸（Glu）及其受体（NMDAR1）的影响,探讨盐酸戊乙奎醚脑保护作用机制。方法：雄性Wistar大鼠60只,随机分为3组（n=20）：假手术组（A组）;缺血再灌注对照组（B组）;盐酸戊乙奎醚干预组,采用Pulsinelli-Brierley四血管阻断法制备全脑缺血模型,实验分两部分进行,各组随机选择10只大鼠于全脑缺血15min再灌注1h、3h、6h,采用脑微透析技术结合高效液相色谱（HPLC）检测大鼠海马细胞外Glu水平的变化,其余10只大鼠于再灌注3h后灌流固定断头取脑,采用免疫组织化学方法,检测海马CA1区NMDAR1蛋白表达。结果：与缺血／再灌注组各对应时点相比较,盐酸戊乙奎醚干预组大鼠海马细胞外Glu含量明显降低,统计结果差异均有显著性（P〈0．05或P〈0．01）;海马CA1区NMDAR1表达明显受抑制,积分光密度、阳性细胞面积、平均灰度值均存在显著性差异（P〈0．05或P〈0．01）。结论：脑缺血／再灌注早期应用盐酸戊乙奎醚不仅减少兴奋性氨基酸释放,还能抑制NMDAR1的高表达而产生脑保护作用。
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| 关 键 词： | 盐酸戊乙奎醚 缺血／再灌注损伤 谷氨酸 NMDA受体 |
Penehyclidine hydrochloride inhibits glutamate release and related research in global brain ischemia/reperfusion rats |
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| Shang Y,Gu PF,Shang Y,Li Y.Penehyclidine hydrochloride inhibits glutamate release and related research in global brain ischemia/reperfusion rats[J].Chinese Journal of Applied Physiology,2011,27(3):353-356. |
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| Authors: | Shang You Gu Pei-fei Shang Yu Li Yue |
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| Institution: | SHANG You1,GU Pei-fei2,SHANG Yu3,LI Yue4(1.Department of Anesthesiology,The First Affiliated Hospital of Liaoning Medical College,Jinzhou 121001,China,2.Department of Physiology,Shenyang Pharmaceatical University,Shenyang 110016,3.Department of Anesthesiology,PLA 463 Hospital,Shenyang 110042,4.Department of Anesthesiology,The First Affiliated Hospital of Harbin Medical University,Haerbin 150001,China) |
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| Abstract: | Objective:To investigate the effect of penehyclidine hydrochloride on glutamate(Glu)release and N-methyl-D-aspartate receptor(NMDAR)1 expression in hippocampus CA1 with global cerebral ischemia/reperfusion rats.Methods: Sixty male Wistar rats were randomly allocated into three groups;group A received sham operation;group B received ischemia/reperfusion;group C received penehyclidine hydrochloride treatment(2 mg/kg) before ischemia/reperfusion(n=20).Global cerebral ischemia was induced according to Pulsinell... |
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| Keywords: | penehyclidine hydrochloride ischemia/reperfusion injury glutamate N-methyl-D-aspartate receptor |
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