杭白菊总黄酮对衰老小鼠学习记忆的影响

目的：探讨杭白菊总黄酮（TFCM）对衰老小鼠学习记忆能力及胆碱能系统的影响。方法：采用皮下注射D-半乳糖的方法制备衰老小鼠模型。ICR小鼠随机分为5组（n=10）：正常对照组,模型组,低、中、高剂量TFCM处理组。TFCM处理组于造模后第2周开始每天给予TFCM（50、100或150mg／kg）灌胃。用Morris水迷宫法检测小鼠学习记忆能力,用比色法测定血清和大脑组织中丙二醛（MDA）含量,以及超氧化物歧化酶（SOD）和乙酰胆碱酯酶（Ach E）活性。结果：与正常对照组相比,模型组学习记忆能力减退,MDA含量和Ach E活性增加,SOD活性降低。与模型组相比,中高浓度TFCM处理组（100、150mg／kg）小鼠学习记忆能力明显改善,MDA含量和Ach E活性明显降低,SOD活性增高。结论：TFEM能显著改善D-半乳糖衰老小鼠学习记忆能力,其机制可能与TFCM的抗氧化特性以及提高中枢胆碱能系统功能有关。

Effect of total flavonoids from chrysanthemun morifolium on learning and memory in aging mice

Objective： To investigate the effect of total flavonoids from Chrysanthemun morifolium（TFCM） on learning and memory, and cholinergic system function in aging mice. Methods： The aging mice model was established by subcutaneous injection of D-galactose. ICR mice were divided into five groups（n= 10）： control group, model group, and TFCM groups. Mice in TFCM groups were given TFCM （50,100 or 150 mg/kg） by gastric irrigation once a day. Learning and memory ability were evaluated by Morris water maze test. The MDA content, SOD and Ach E activity were also measured. Results： Compared with control group, learning and memory ability declined in the D-galactoseinduced aging mice; meanwhile MDA content and AchE activity increased, SOD activity decreased. Treatment with TFCM （ 100, 150 mg/kg） ameliorated the decrease in learning and memory ability of aging mice. Compared with model group, TFCM（ 100, 150 mg/kg） could also decrease MDA content and Ach E activity, and increase SOD activity in aging mice. Conclusion： TFCM may improve the learning and memory ability of aging mice. The mechanism is involved in its antioxidative characteristic and improvement of central cholinergic system function.