Some Properties of the Lipase of Geotrichum candidum Evaluated by a Fluorimetric Assay Technique |
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| Authors: | ADRIANA M P DOOIJEWAARD-KLOOSTERZIEL J T M WOUTERS |
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| Institution: | Laboratory for Microbiology, University of Amsterdam, Plantage Muidergracht 14, Amsterdam, The Netherlands |
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| Abstract: | The oleic acid ester of 4-methylumbelliferone was found to be a suitable substrate for the fluorimetric assay of the lipase of Geotrichum candidum . This substrate spontaneously formed an emulsion in the assay mixture and had a K m of 10·3 μM. The fluorimetric method was at least a thousand times more sensitive than the conventional potentiometric method. The lower limit of sensitivity of the assay was 1 nmole fatty acid released per min. Optimum conditions for detection of lipase in the culture filtrate of G. candidum were devised. The influence of pH, Ca2+ and several other factors were investigated. |
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