Studies of the Kinetochore Proteins of the Regenerating Liver and the Liver Cells of Rats at Different Stages of Development

The kinetochore composition of rat liver cells was studied by indirect immunofluorescence and immunoblotting using human anti-kinetochore/centromere autoantibodies(ACAs).Besides three major antigens(50kD,42 kD and 34 kD),ACAs used in this study could also identify those of 32- 30 kD and 20 kD in newborn rat liver cells,90 kD in old rat liver cells,37 kD and 32-30 kD in regenerating liver cells.These results indicate that some kinetochore antigen(s)may be related to cell proliferation or specific for different stages of development.