| 空间搭载高产葡萄糖耐量因子(GTF)酵母的选育 |
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| 引用本文： | 刘 鹭,吕加平,高艳红.空间搭载高产葡萄糖耐量因子(GTF)酵母的选育[J].微生物学通报,2009,36(2):0223-0230. |
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| 作者姓名： | 刘 鹭 吕加平 高艳红 |
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| 作者单位： | 1. 中国农业科学院农产品加工所,北京,100193
2. 内蒙古农业大学食品科学与工程学院,内蒙古,呼和浩特,010018 |
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| 基金项目： | 国家航天育种工程(No. 2006HT00013) |
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| 摘 要： | 以从本试验室收藏的7株酵母菌种及其7株经空间搭载(实践八号)诱变后的酵母菌株为研究对象, 通过梯度含铬YEPD平板培养和液体YPD培养基发酵试验, 利用氨水提取菌体中的GTF和火焰原子吸收光谱法检测其有机铬含量, 从中筛选出一株葡萄糖耐量因子的高产酵母(YS-3), 有机铬含量达1296 mg/g, 总铬含量达1926 mg/g, 生物量为40 g/L。同时分析了菌体发酵过程中, 菌体有机铬富集量与其它发酵参数的动态关系。
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| 关 键 词： | 空间搭载 葡萄糖耐量因子 GTF 有机铬 |
Selection of a High Yield Yeast Strain of Glucose Tolerance Factor After Space Piggyback Experiment |
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| LIU Lu,LV Jia-Ping and GAO Yan-Hong.Selection of a High Yield Yeast Strain of Glucose Tolerance Factor After Space Piggyback Experiment[J].Microbiology,2009,36(2):0223-0230. |
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| Authors: | LIU Lu LV Jia-Ping and GAO Yan-Hong |
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| Institution: | Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China;Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China;College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010018, China |
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| Abstract: | A strain of high-biomass, producing glucose tolerance factor yeast strain was screened from seven yeast strains and seven strains mutated by spaceflight-induced mutagenesis through gradient concentration of chromium plates cultivation and liquid fermentation cultivation. The biomass of it was 40 g/L. Extracting GTF with ammonia and determining the content of Cr with flame atomic absorption spectrometry, the content of organic Cr was 1287 mg/g and that of total Cr was 1917 mg/g. At the same time, dynamic relationship between other parameters and the amount of organic chromium was analyzed during fermentation. |
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| Keywords: | Space piggyback experiment Glucose tolerance factor GTF Organic chromium |
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