| 外源激素对番茄质膜结合植物凝集素的影响 |
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| 引用本文： | 林忠平,崔庆玲. 外源激素对番茄质膜结合植物凝集素的影响[J]. Acta Botanica Sinica, 1985, 0(3) |
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| 作者姓名： | 林忠平 崔庆玲 |
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| 作者单位： | 中国科学院植物研究所，中国科学院植物研究所 |
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| 摘 要： | 番茄愈伤组织培养于附加不同激素的 MS 培养基上。经过3次继代培养后,分别从这些愈伤组织分离原生质体,并使之与植物凝集素 ConA 结合。再从原生质体制备质膜。比较在相同膜蛋白含量条件下,质膜上结合~(125)I-ConA。结果表明:在含有2,4-D 的培养基上番茄培养细胞的质膜结合 ConA 的能力保持在很高的水平上。其它生长物质和激动素对细胞表面特性的影响各不相同。
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| 关 键 词： | 番茄 愈伤组织 原生质体 质膜 外源激素 ~(125)Ⅰ-ConA |
THE EFFECT OF EXOGENOUS HORMONES ON CONCANAVALIN A BINDING BY THE PLASMA MEMBRANE OF TOMATO |
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| Lin,Zhong-ping Cui,Qing-ling. THE EFFECT OF EXOGENOUS HORMONES ON CONCANAVALIN A BINDING BY THE PLASMA MEMBRANE OF TOMATO[J]. , 1985, 0(3) |
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| Authors: | Lin Zhong-ping Cui Qing-ling |
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| Abstract: | The tomato calli were grown on the MS media supplemented with dif- ferent kinds of exogenous hormones respectively and the protoplasts were isolated from them after subculturing for 3 times.The concanavalin A (Con A) binding capacities of plasma membranes prepared from the protoplasts were compared by using radioche- mical assay with~(125)I-Con A.The result shows that Con A reactivity of plasma memb- rane is greatly increased by 2,4-D (2 mg/l) treatment and the other growth substances affect on Con A binding capacity variously. |
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