Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins |
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| Authors: | P E Reid D A Owen C W Ramey W L Dunn E A Jones D A Lazosky E Allen C M Park and M G Clay |
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| Institution: | (1) Department of Pathology, Faculty of Medicine, University of British Columbia, V6T 1W5 Vancouver, British Columbia, Canada;(2) Department of Medical Genetics, Faculty of Medicine, University of British Columbia, V6T 1W5 Vancouver, British Columbia, Canada;(3) Department of Surgery, Faculty of Medicine, University of British Columbia, V6T 1W5 Vancouver, British Columbia, Canada |
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| Abstract: | Summary A differential diagnostic scheme is described for the division of colonic epithelial glycoproteins into eleven histochemically distinct classes. The scheme depends upon the use of seven histochemical techniques which, collectively, permit the differential staining ofO-sulphate ester, sialic acid and its side chainO-acyl variants and vicinal diols located on carbohydrate residues other than sialic acids. Elements of the scheme also provide a general approach to the classification of epithelial glycoproteins in anatomic sites other than the colon.Application of the scheme permitted the classification of the epithelial glycoproteins in the mucosa 0.5–5.0 cm from human colonic tumours and provided direct confirmation of previous observations that changes from normal in the relative proportions of either side chainO-acylated sialic acids or sialic acids andO-sulphate esters can occur independently of one another. |
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