Isolation and characterization of chromium(VI)-reducing Bacillus sp. FY1 and Arthrobacter sp. WZ2 and their bioremediation potential |
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| Authors: | Wendan Xiao Xuezhu Ye Xiaoe Yang Zhiqiang Zhu Caixia Sun Qi Zhang |
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| Affiliation: | 1. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;2. Ministry of Education Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China;3. College of Agriculture, Hainan University, Haikou, China |
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| Abstract: | Two native bacterial strains, FY1 and WZ2, that showed high chromium(VI)-reducing ability were respectively isolated from electroplating and tannery effluent–contaminated sites and identified as Bacillus and Arthrobacter. The objective of the present study was to evaluate their potential for future application in soil bioremediation. The results showed that both Bacillus sp. FY1 and Arthrobacter sp. WZ2 were tolerant to 1000 mg L?1 Cr(VI) and capable of reducing 78–85% and 75–82% of Cr(VI) (100–200 mg L?1) within 24 h, respectively. The Cr(VI) reduction rate decreased with increasing levels of Cr(VI) concentration (200–1000 mg L?1). The optimum pH, temperature, and inoculum concentration for Cr(VI) reduction were found to be between pH 7.0 and 8.0; 30 and 35°C; and 1 × 108 cells ml?1, respectively. Further evidence for the bioremediation potential of Bacillus sp. FY1 and Arthrobacter sp. WZ2 was provided by the high capacity to reduce 100, 200, and 500 mg kg?1 Cr(VI) in contaminated soil by 83–91%, 78–85%, and 71–78% within 7 days, respectively. These findings demonstrated the high potential of Bacillus sp. FY1 and Arthrobacter sp. WZ2 for application in future soil bioremediation. |
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| Keywords: | Arthrobacter Bacillus bioremediation potential chromium-reducing bacteria chromium resistance |
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