Restriction and modification in Bacillus subtilis Marburg 168: target sites and effects on plasmid transformation |
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Authors: | S Bron L Jannière and S D Ehrlich |
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Institution: | (1) Department of Genetics, Center of Biological Sciences, Kerklaan 30, 9751 NN Haren (Gn), The Netherlands;(2) Institut Jacques Monod, Tour 43, 2 Place Jussieu, F-75251 Paris Cedex 05, France |
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Abstract: | Summary The effects of the restriction system of Bacillus subtilis strain M on plasmid transformation were studied. Plasmid pHV1401 DNA prepared from B. subtilis transformed the restriction-proficient M strain 100 times more efficiently than the DNA prepared from Escherichia coli, while the two DNA preparations transformed restriction-deficient derivatives of that strain with similar efficiencies. This indicates that transformation with pHV1401 is sensitive to the M restriction system. pHV1401 contains three CTCGAG (XhoI sites). Successive removal of these abolished the effect of restriction. This indicates that the XhoI sites are the targets for the M restriction system.Abbreviations used Apr
resistance to ampicillin
- Cmr
resistance to chloramphenicol
- R/M
restriction and modification
- Tcr
resistance to tetracycline |
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Keywords: | Plasmid transformation BsuM restriction pHV1401 XhoI sites |
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