| Affiliation: | (1) Present address: Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, 244-0813 Yokohama, Japan;(2) PE Biosystems Japan Ltd., 279-0011 Chiba, Japan;(3) Graduate School of Human and Environmental Studies, Kyoto University, 606-8501 Kyoto, Japan;(4) Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, 603-8047 Kyoto, Japan;(5) Research Institute for Bioresources, Okayama University, 710-0046 Kurashiki, Japan;(6) Laboratory of Plant Genetics, Department of Biological and Environmental Science, Faculty of Agriculture, Kobe University, 657-8501 Kobe, Japan;(7) Center for Information Biology, National Institute of Genetics, 411-8540 Mishima, Japan;(8) Department of Bioscience, Fukui Prefectural University, 910-1195 Fukui, Japan |
| Abstract: | Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat,Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA—a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request. |
