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带芒草属低分子量谷蛋白基因的克隆及序列分析
引用本文:曾节,代寿芬,郑有良,刘登才,魏育明,颜泽洪. 带芒草属低分子量谷蛋白基因的克隆及序列分析[J]. 遗传, 2008, 30(5): 633-641. DOI: 10.3724/SP.J.1005.2008.00633
作者姓名:曾节  代寿芬  郑有良  刘登才  魏育明  颜泽洪
作者单位:四川农业大学小麦研究所, 都江堰 611830
基金项目:教育部跨世纪优秀人才培养计划,教育部全国优秀博士学位论文作者专项基金,国家自然科学基金,国家高技术研究发展计划(863计划)
摘    要:在普通小麦中获得了大量的低分子量谷蛋白基因序列, 而在小麦近缘属物种中获得的同源基因则比较少, 导致对麦类低分子量谷蛋白基因家族成员间的关系还不清楚。因此, 进行近缘属物种低分子量谷蛋白基因的研究是非常必要的。此研究通过特殊设计的1对引物, 以小麦近缘属带芒草物种的基因组DNA为模板, 经过PCR和克隆, 从中得到了一条核苷酸序列长度为1 035 bp, 推测的氨基酸序列为343个氨基酸残基的低分子量谷蛋白基因, 该基因序列具有小麦低分子量谷蛋白基因的典型特征, 包括21个氨基酸残基的信号肽、13个氨基酸的N-端和由可重复的短肽单元组成的重复区以及1个C末端。序列比对结果揭示了来自带芒草的低分子量谷蛋白基因与小麦同源基因的差异及相互关系。此研究结果对从带芒草属以及其他小麦近缘属物种中分离未知低分子量谷蛋白基因有参考价值和借鉴意义。

关 键 词:序列分析  基因克隆  低分子量谷蛋白  带芒草属  
收稿时间:2007-10-28
修稿时间:2007-10-28

Isolation and characterization of a low molecular weight glutenin gene from Taenitherum Nevski
ZENG Jie,DAI Shou-Fen,ZHENG You-Liang,LIU Deng-Cai,WEI Yu-Ming,YAN Ze-Hong. Isolation and characterization of a low molecular weight glutenin gene from Taenitherum Nevski[J]. Hereditas, 2008, 30(5): 633-641. DOI: 10.3724/SP.J.1005.2008.00633
Authors:ZENG Jie  DAI Shou-Fen  ZHENG You-Liang  LIU Deng-Cai  WEI Yu-Ming  YAN Ze-Hong
Affiliation:Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China
Abstract:More and more low-molecular-weight glutenin(LMW glutenin) genes were isolated and characterized from hexaploid wheat (Triticum aestivum L.). However, few homologous genes were obtained from its relative species, which limited our understanding of the relationships among them. Therefore, it is necessary to isolate LMW glutenin homologous genes from wheat wild relative species. Using a pair of specific oligonucleotide PCR primers for Taenitherum genomic DNA, a LMW glutenin gene sequence, with nucleotide sequence in 1 035 bp and deduced amino acid sequence with 343 amino acid residues, was obtained. This sequence was a typical LMW glutenin sequence and characterized by a signal peptide of 21 amino acid residues, a N-terminal conservative domain of 13 amino acid residues, a repetitive domain of short peptide, and a C-terminal conservative domain. Sequence alignment showed the main differences and the relationships between LMW glutenin genes from wheat and Taenitherum. The results presented here give a reference to isolate LMW glutenin gene from Taenitherum, as well as other wheat wild relatives.
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