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A reliable protocol for direct detection of lectin binding sites on the plasma membrane of a single living sperm cell in maize |
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| Authors: | Meng-Xiang Sun Ehard Kranz Alessandra Moscatelli Hong-Yuan Yang Horst Lörz Mauro Cresti |
| Affiliation: | The key laboratory of MOE for Developmental Biology, Wuhan University, Wuhan 430072, PR China, Center for Applied Plant Molecular Biology, AMP II, Institute for General Botany, University of Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany, Department of Biology, Milan University, Via Celoria 26, 20133 Milan, Italy, Department of Environmental Biology, Siena University, 53100 Siena, Italy, |
| Abstract: | . A novel protocol for direct detection and localization of lectin binding sites on the surface of single sperm cells is presented. Fluorescence-conjugated lectins and single cell manipulation techniques were employed in this protocol. Advantages of the protocol include that sperm cell membranes are well protected and artifacts avoided by minimizing movement of the medium. Damage of the sperm membrane was reduced using a droplet-staining/washing method. Any morphological change may be readily recorded. |
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