Serine/threonine protein phosphatases in Dictyostelium discoideum: no evidence for type I activity. |
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Authors: | M N Simon T Winckler R Mutzel M Véron J C da Costa Maia |
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Affiliation: | Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Brazil. |
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Abstract: | Extracts from Dictyostelium discoideum contain type 2A and 2C serine/threonine-specific protein phosphatases with properties very similar to those from mammals according to their sensitivity to okadaic acid and to their dependence for divalent cations. In contrast, no type 1 protein phosphatase is found at any time of development, neither in the cytosolic nor in the particulate fraction, using glycogen phosphorylase a, casein, histone or the non-proteinous 4-Methylumbelliferyl phosphate as substrates. Both type 2A and 2C protein phosphatase activities remain constant throughout the development cycle. |
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