Detection of Streptococcus anginosus from saliva by real-time polymerase chain reaction |
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Authors: | Kumagai K Sugano N Takane M Iwasaki H Tanaka H Yoshinuma N Suzuki K Ito K |
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Affiliation: | Department of Periodontology, Nihon University School of Dentistry, Kanda-Surugadai, Chiyoda-ku, Tokyo, Japan. |
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Abstract: | AIMS: The purpose of the present investigation was to assess the salivary levels of Streptococcus anginosus in periodontitis patients. METHODS AND RESULTS: The salivary levels of Strep. anginosus were assessed using real-time polymerase chain reaction (PCR). Streptococcus anginosus was detected in 28 of 37 (75.6%) of periodontitis patients and in three of the 20 (15%) healthy subjects. The mean values for bleeding on probing and probing depth in positive patients were statistically higher than those in negative patients. A significant decrease in Strep. anginosus levels was observed after periodontal treatment. CONCLUSIONS: Although the levels of Strep. anginosus are extremely low, they may reflect the status of periodontal health. SIGNIFICANCE AND IMPACT OF THE STUDY: Real-time PCR is a useful method for obtaining the relative quantities of Strep. anginosus from saliva samples and for monitoring the effect of therapy. |
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Keywords: | periodontitis real-time PCR saliva Streptococcus anginosus |
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