Placental Alpha Hemoglobin Stabilizing Protein (AHSP) and recurrent miscarriage |
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Authors: | Monica Emanuelli Monia Cecati Davide Sartini Piergiorgio Stortoni Alessandra Corradetti Stefano R Giannubilo Angelo Turi Andrea L Tranquilli |
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Institution: | (1) Institute for Biochemical Biotechnologies, Università Politecnica delle Marche, Ancona, Italy;(2) Institute for Maternal and Child Sciences, Università Politecnica delle Marche, Via Corridoni 11, 60123 Ancona, AN, Italy |
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Abstract: | AHSP inhibits cellular production of the reactive oxygen species. Reduced AHSP indicates reduced protection against oxidative
stressors. Our objective was to investigate AHSP levels in recurrent miscarriage (RM). Trophoblast was collected from women
of 10 weeks gestation: voluntary abortion controls (VA, n = 10); spontaneous first miscarriage with subsequent normal pregnancy (SMSN, n = 15) or with subsequent miscarriage (SMSM, n = 5); RM previously investigated (RMPS, n = 5) or not previously investigated (RM, n = 5). AHSP mRNA and protein were determined using real-time quantitative polymerase chain reaction (PCR) and Western blot,
respectively. One-way ANOVA was performed to assess statistical significance (p < 0.05). ahsp mRNA levels were maximally reduced in RM and RMPS (8.0 × 10−6 ± 1.3 and 8.1 × 10−6 ± 0.7, respectively) compared with SMSN and VA (16.1 × 10−6 ± 2.3 and 26.1 × 10−6 ± 2.7, respectively). SMSM showed levels significantly reduced as well (9.0 × 10−6 ± 2.3). In RM, a reduced defense from oxidative stressors is evident at first miscarriage, identifying women at high risk
for subsequent eventful pregnancy. Reduced AHSP may identify women at risk of experiencing further miscarriages.
Monica Emanuelli and Monia Cecati contributed equally to this paper. |
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Keywords: | AHSP Recurrent miscarriage Trophoblastic tissue |
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