| 新基因 P15RS 表达产物在人胃癌细胞细胞周期中定位的研究 |
| |
| 引用本文： | 张小勇,张 伟,高 萍,常智杰,孙一娜,柳惠图. 新基因 P15RS 表达产物在人胃癌细胞细胞周期中定位的研究[J]. 生物化学与生物物理进展, 2005, 32(8): 771-776 |
| |
| 作者姓名： | 张小勇 张 伟 高 萍 常智杰 孙一娜 柳惠图 |
| |
| 作者单位： | 1. 北京师范大学生命科学学院,细胞增殖及调控生物学教育部重点实验室,北京,100875
2. 清华大学生命科学与医学研究院,北京,100084 |
| |
| 基金项目： | 国家自然科学基金，教育部实验室基金 |
| |
| 摘 要： | P15RS是在p15INK4b高表达的人黑色素瘤细胞MLIK6 G1期中发现和克隆的新基因.研究表明,该基因可能在G1期作为增殖负调因子起作用.为了观察P15RS基因产物在细胞中的定位,构建了表达P15RS-EGFP融合蛋白的真核表达质粒pEGFP-P15RS.用绿色荧光蛋白GFP作为报告基因,以人胃癌细胞BGC-823为实验模型,将pEGFP-P15RS转染BGC-823细胞.实验表明,P15RS基因表达产物在G1期、S期和G2期均定位于细胞核内,未见在核仁中分布,M期凝缩的染色体上未见.因此,P15RS可能定位在核质中.
|
| 关 键 词： | 人胃癌细胞 细胞定位 |
| 收稿时间： | 2005-02-25 |
| 修稿时间： | 2005-02-252005-03-31 |
Protein Localization of A Novel Gene P15RS in Cell Cycle of BGC-823 Cells |
| |
| ZHANG Xiao-Yong,ZHANG Wei,GAO Ping,CHANG Zhi-Jie,SUN YI-NA and LIU Hui-Tu. Protein Localization of A Novel Gene P15RS in Cell Cycle of BGC-823 Cells[J]. Progress In Biochemistry and Biophysics, 2005, 32(8): 771-776 |
| |
| Authors: | ZHANG Xiao-Yong ZHANG Wei GAO Ping CHANG Zhi-Jie SUN YI-NA LIU Hui-Tu |
| |
| Affiliation: | Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science, Beijing Normal University, Beijing 100875, China;Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science, Beijing Normal University, Beijing 100875, China;Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science, Beijing Normal University, Beijing 100875, China;Institute of Biomedicine, Tsinghua University, Beijing 100084, China;Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science, Beijing Normal University, Beijing 100875, China;Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science, Beijing Normal University, Beijing 100875, China |
| |
| Abstract: | P15RS was identified as a novel gene cloned from the human melanoma cell model MLIK6 overexpressing p15INK4b. P15RS has been reported as a negative regulator of cell proliferation in G1 phase. To investigate the localization of P15RS, EGFP-P15RS, a fusion protein with EGFP reporter was constructed and overexpressed in BGC-823 cells. The results showed that EGFP-P15RS protein was distributed in nuclear in interphase (G1, S, G2 phase) of BGC-823 cells. The EGFP-P15RS protein was observed not to localize in chromosome in M phase, suggesting that P15RS might be located in karyoplasm. |
| |
| Keywords: | P15RS GFP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
|
点击此处可从《生物化学与生物物理进展》下载全文 |
|
