Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy |
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| Authors: | Galli Paolo Strona Giovanni Villa Anna Maria Benzoni Francesca Fabrizio Stefani Doglia Silvia Maria Kritsky Delane C |
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| Institution: | Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. |
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| Abstract: | A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy. |
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