Quantitative study of enzyme immunocytochemical reactions performed with enzyme conjugates immobilized on nitrocellulose |
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| Authors: | P H Nibbering J G J Marijnen A K Rapp P C J Leijh R van Furth |
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| Institution: | (1) Department of Infectious Diseases, University Hospital, P.O. Box 9600, NL-2300 RC Leiden, The Netherlands;(2) Department of Histochemistry and Cytochemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, NL-2300 RA Leiden, The Netherlands |
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| Abstract: | Summary The nitrocellulose binding assay was used for quantitative studies on the cytochemical reactions for the three enzymes most frequently used in immunocytochemistry. The results show a linear relationship between the amount of enzyme immobilized on nitrocellulose and the amount of the enzyme reaction product. The similar course of the formation of the reaction product after DAB/H2O2 staining for peroxidase immobilized on nitrocellulose and for immunoperoxidase labeled cells indicates a linear relationship between the amount of enzyme-coupled antibodies bound to cells and the amount of enzyme reaction product. Furthermore, a mild acid treatment for the abolition of endogeneus peroxidase activity in tissues and cells applicable to immunoperoxidase staining procedures is proposed.In honour of Prof. P. van Duijn |
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