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Utilization of tmRNA sequences for bacterial identification |
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| Authors: | Wilhelm Schönhuber Guenhael Le Bourhis Josselyne Tremblay Rudolf Amann Saulius Kulakauskas |
| Affiliation: | 1. Lehrstuhl für Mikrobielle ?kologie,Fach M654, Universit?t Konstanz, Universit?tsstrasse 10, D-78457, Konstanz, Germany 4. Max-Planck-Institut für Marine Mikrobiologie, Celsiusstrasse 1, D-28359, Bremen, Germany 2. Unité de Recherches Laitières et de Génétique Appliquée,Domaine de Vilvert, Institut National de la Recherche Agronomique, 78352, Jouy-en-Josas, France 3. Laboratoire de Pharmacologie des Agents Anticancéreux, Institut Bergonié, 229, Cours de l'Argonne, 33076, Bordeaux, France |
| Abstract: | BackgroundRibosomal RNA molecules are widely used for phylogenetic and in situ identification of bacteria. Nevertheless, their use to distinguish microorganisms within a species is often restricted by the high degree of sequence conservation and limited probe accessibility to the target in fluorescence in situ hybridization (FISH). To overcome these limitations, we examined the use of tmRNA for in situ identification. In E. coli, this stable 363 nucleotides long RNA is encoded by the ssrA gene, which is involved in the degradation of truncated proteins. |
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