Agitation effects on microcarrier and suspension CHO cells |
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| Authors: | Kim C. O’Connor E. Terry Papoutsakis |
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| Affiliation: | (1) Chemical Engineering Department, Tulane University, 70118 New Orleans, LA, USA;(2) Chemical Engineering Department, Northwestern University, 60208 Evanston, IL, USA |
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| Abstract: | Summary The growth properties of attachment-dependent and attachment-independent CHO cells cultured in spinner-flask bioreactors were compared to investigate cell damage from rapid agitation. Damage was attributed to bulk-fluid turbulence for attachment-dependent CHO cells and bubble breakup associated with vortex formation for attachment-independent CHO cells. Radiolabeling demonstrated that cell damage included intrinsic changes in DNA synthesis. |
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