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A rapid method for staining semithin tissue sections embedded in araldite
Authors:E V Loginov  Iu Ia Sokolova  A Ia Gromov
Abstract:A new rapid method is proposed for staining of semithin sections. The method involves the treatment of sections with a methylene blue solution with a slight heating for 1-3 minutes. The method allows to receive polychromatic contrast preparations, to save time and reagents. It also permits to avoid restaining effect. The preparations can be preserved for a long time in plastic media (e.g. in polystyrene) for light microscopy. A comparative analysis of the staining methods used in the electron microscopic practice is given.
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