Free-radical first responders: The characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica |
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| Authors: | Neal J. Dawson Barbara A. KatzenbackKenneth B. Storey |
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| Affiliation: | Department of Biology and Institute of Biochemistry Carleton University, Ottawa, ON, Canada |
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| Abstract: | |
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| Keywords: | ALS, amyotrophic lateral sclerosis AMPK, 5&prime adenosine monophosphate-activated protein kinase Cm, the denaturant concentration at which half of the protein is unfolded CuZnSOD, copper and zinc containing SOD DEAE+, diethylaminoethyl cellulose DEPC, diethylpyrocarbonate DTT, dithiothreitol FeSOD, iron containing superoxide dismutase Km, Michaelis&ndash Menten constant IMAC, immobilized metal ion affinity chromatography IP3, inositol trisphosphate KPi, potassium phosphate MAPK, mitogen activated protein kinase MnSOD, manganese containing superoxide dismutase NBT, nitrotetrazolium blue NiSOD, nickel containing superoxide dismutase PKA, protein kinase A PKC, protein kinase C PKG, protein kinase G PMSF, phenylmethylsulfonyl fluoride PP1, protein phosphatase 1 PP2A, protein phosphatase 2A PP2C, protein phosphatase 2C PVDF, polyvinylidene difluoride ROS, reactive oxygen species TBST, Tris buffered saline with Tween Vmax, maximal velocity |
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